Enzyme Lab Worksheet

Hypothesis:  My hypothesis is that the acidic solution will speed up and increase the process, thus creating more bubbles.

Independent Variable:  The pH level of the solution and the amount that we add to the bindweed solution.

Dependent Variable:  The amount of bubbles produced by the mixture once the hydrogen peroxide is added.

Controlled Variables:  Amount of the different solutions and the acidity of them that we add.

Justification of hypothesis:  The low pH level burns skin so I thought it would kill the enzymes.

Why did you choose this as your hypothesis?

Materials (Your Team’s Experiment):  test tube, metric ruler, stopwatch (iphone), bindweed, hydrogen peroxide, sodium hydroxide, hydrogen peroxide, water, mortar and pestle, 1 milliliter pipettes, beakers.

Procedure:

1. Mash up one handful of bindweed with 30 ml of water in a mortar and pestle
2. filter using a paper towel into a separate glass, throw away paper towel
3. tape ruler, metric side, to test tube
4. extract 1 ml of bindweed solution into test tube
5. extract .7 ml of hydrogen peroxide into same test tube
6. observe bubbles and and time the rate of formation
7. record information
8. with a new test tube repeat steps 1-4
9. add .5 ml of hydrochloric acid to the bindweed solution
10. repeat step 5-7
11. with a new test tube repeat steps 1-4
12. add .5 ml of sodium hydroxide to bindweed solution
13. repeat steps 5-7
14. write out lab report
15. clean up lab space

Summary:  When we tested all of our different solutions, we realized that the control actually created the biggest reaction and the most bubbles.

Data and Results:

Conclusions:  My hypothesis was wrong neither variable created more bubbles.